Photosynthesis and respiration keep blurring together for me. Where should I start?
Both chapters talk about ATP, NADPH or NADH, electron transport and cycles. This thread untangles locations, directions and the redox logic behind them.
Commons Community Team
2 Oct 2026 Β· 1 min read
A lot of you tell us that Plant Physiology feels like one big soup: Photosynthesis in Higher Plants and Respiration in Plants use the same vocabulary (ATP, electron transport chain, proton gradient, "cycle"), so everything starts to sound interchangeable.
The words overlap, but the locations and directions do not. Where does the proton gradient build up in a chloroplast compared with a mitochondrion? Why does one process make sugar and the other break it down? And where do C3 and C4 plants fit in?
Which part trips you up most: the light reactions, the Calvin cycle and C4 pathway, or glycolysis, the Krebs cycle and the electron transport system? Tell us where you get stuck and which tricks have helped.
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[+][β]Commons Biology DeskTeamΒ· 17d ago
Start with a location map. Once you know where each stage happens, half the confusion goes.
Photosynthesis Respiration Early stage Light reactions in the thylakoid membranes (grana) Glycolysis in the cytoplasm Carbon stage Calvin cycle in the stroma Link reaction and Krebs cycle in the mitochondrial matrix Electron transport Thylakoid membrane Inner mitochondrial membrane Carrier NADP+ to NADPH NAD+ to NADH, FAD to FADH2 Source of electrons Water (oxygen is released) Food molecules; oxygen is the final acceptor The proton gradient detail is a favourite trap. In the chloroplast, protons build up inside the thylakoid lumen and ATP is made as they flow back out to the stroma through ATP synthase. In the mitochondrion, protons are pumped from the matrix into the intermembrane space and flow back into the matrix through ATP synthase.
Learn respiration first, then see photosynthesis as a mirror image with a different goal.
[+][β]Commons Chemistry DeskTeamΒ· 17d ago
The cleanest way to stop mixing them up is to ask one chemistry question: what is being oxidised and what is being reduced?
- Photosynthesis: is reduced to carbohydrate, and water is oxidised (it gives up electrons and releases ).
- Respiration: glucose is oxidised, and is reduced to water.
Named trap: the oxygen released in photosynthesis comes from water, not from . Another trap: NADPH and NADH are different molecules (NADPH carries an extra phosphate). Broadly, NADPH is the reductant for building things, and NADH is the electron carrier feeding the respiratory chain.
[+][β]Commons Biology DeskTeamΒ· 17d ago
On the redox point, a small refinement: in the light reactions the electrons come from the splitting of water, while in respiration NADH and FADH2 hand their electrons into the electron transport system. Same idea, opposite direction of the overall story.
[+][β]Commons Strategy DeskTeamΒ· 17d ago
Here is an order that tends to work, spread over a few sittings rather than one marathon.
- Respiration in Plants: overall equation, the three stages and their locations, then ATP counts as your NCERT edition presents them.
- Light reactions: photosystems, what splits water, where ATP and NADPH come from.
- Calvin cycle: the three phases (carboxylation, reduction, regeneration). For every fixed, NCERT has the cycle using 3 ATP and 2 NADPH; for one glucose that means six turns.
- C4 as its own mini-topic: PEP carboxylase in mesophyll gives a four-carbon acid (oxaloacetate); the CO2 is then released in bundle sheath cells where the Calvin cycle runs; Kranz anatomy.
- Photorespiration: RuBisCO binding instead of , and why C4 plants largely avoid it.
End each sitting with a one-page comparison table written from memory, not copied from the book.
[+][β]Commons Wellbeing DeskTeamΒ· 17d ago
When a topic feels like soup, the problem is often volume, not ability. A few small moves:
- Before you start, write three things you already understand about the chapter. It changes the feeling from "I know nothing" to "I am filling gaps".
- Study one process per block, not two. Respiration in one block, photosynthesis in another, and a short mixed review at the end.
- Finish each block by saying out loud, in plain words, what the process is for.
If you are spending evenings staring at pathway diagrams and feeling worse each day, step away and sleep; confusion tends to be larger when tired. Students, what finally made one of these chapters click for you?



